By Leung Kwok-Sui, Ling Qin, Wing-hoi Cheung
This handbook offers technical protocols for musculoskeletal examine on a translational foundation, i.e. a disease-orientated strategy. It bargains tips on numerous laboratory concepts, together with mobilephone tradition and molecular biology, histology and histomorphometry, microscopy and bioimaging, laboratory animal versions, CT- and MRI-based densitometry and microarchitectural research, biomechanics and practical research of orthopedic kinesiology, and so forth. The content material is easy and easy, with illustrations and step by step approaches as a simple experimental reference for group of workers in easy and scientific musculoskeletal study and schooling. This ebook will supply a different multidisciplinary platform for numerous professions -- not just orthopedics, but in addition biomedical engineering and biomaterial sciences -- related to either easy and medical drugs.
Contents: telephone tradition and Molecular Biology; Histology and Histomorphometry; Microscopy and Bioimaging; Laboratory Animal types; X-Ray- and MRI-based Densitometry; Biomechanics and movement research
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Extra info for A Practical Manual for Musculoskeletal Research
A Most of the steps have now been commercialized (and some even robotized). This has resulted in some advantages, such as the use of commercial kits in several steps, giving greater consistency. However, one unfortunate disadvantage is that trainees entering this field may not be aware of the respective roles of all the components of the system, and consequently may not know how to fix or adjust the system when things go wrong. In other words, they may not know what they are doing if they simply follow the kit instructions.
2001; Vaes et al. 2002; De et al. 2002; Balint et al. 2003). We believe that the potential of DNA microarray in human bone studies has yet to be explored, especially in musculoskeletal research, and may dramatically expand our scope of understanding molecular programs underlying the physiological and pathological conditions of musculoskeletal systems. This chapter will focus primarily on detailed protocols of DNA microarrays, in particular expression arrays. 2. Materials For the following materials, alternative vendors can be used, but pay special attention to the selection of microscope slides, reverse transcriptase, and Cy3- and Cy5-labeled oligonucleotides.
Gene expression profiling of bone marrow stromal cells from juvenile, adult, aged and osteoporotic rats: with an emphasis on osteoporosis. Bone 40:700–715, 2007. Zheng PZ, Wang KK, Zhang QY et al. Systems analysis of transcriptome and proteome in retinoic acid/arsenic trioxide combination-induced cell differentiation/apoptosis of acute promyelocytic leukemia. Proc Natl Acad Sci USA 102:7653–7658, 2005.
A Practical Manual for Musculoskeletal Research by Leung Kwok-Sui, Ling Qin, Wing-hoi Cheung